Temperature Control System for Measuring Planar Lipid Bilayer Properties
نویسندگان
چکیده
منابع مشابه
Phosphoinositides alter lipid bilayer properties
Phosphatidylinositol-4,5-bisphosphate (PIP2), which constitutes ∼1% of the plasma membrane phospholipid, plays a key role in membrane-delimited signaling. PIP2 regulates structurally and functionally diverse membrane proteins, including voltage- and ligand-gated ion channels, inwardly rectifying ion channels, transporters, and receptors. In some cases, the regulation is known to involve specifi...
متن کاملVoltage- and Current-Clamp Methods for Determination of Planar Lipid Bilayer Properties
Biological membranes, the barriers that envelope the cell and its inner organelles, play a crucial role in the normal functioning of cells. The simplest model of these biological membranes is the planar lipid bilayer. Because its geometry allows chemical and electrical access to both sides of the bilayer, the physical properties of this model membrane can be easily measured. Usually, a thin bim...
متن کاملRapid Perfusion System for Inhibition Investigation of Membrane Proteins in Planar Lipid Bilayer
This paper describes measurement system of membrane proteins with a rapid perfusion capability using microfluidic channels. In the previous report, we successfully recorded channel conductance of alpha-hemolysine (HL) in bilayer lipid membranes (BLMs) using droplets contacting method with PDWC (parylene double well chip). This method is one of the easiest ways for preparing the BLMs, just pipe...
متن کاملAlcohol's effects on lipid bilayer properties.
Alcohols are known modulators of lipid bilayer properties. Their biological effects have long been attributed to their bilayer-modifying effects, but alcohols can also alter protein function through direct protein interactions. This raises the question: Do alcohol's biological actions result predominantly from direct protein-alcohol interactions or from general changes in the membrane propertie...
متن کاملThe Design and Use of a Planar Lipid Bilayer Workstation
A planar lipid bilayer (BLM) workstation, used to record currents through actively gating, ion conducting single channels, is a complex apparatus requiring several components working in concert. These components include a means to support the lipid membrane, high gain amplification, shielding of electromagnetic interference and mechanical vibration, mechanisms for stirring and changing solution...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Procedia Engineering
سال: 2012
ISSN: 1877-7058
DOI: 10.1016/j.proeng.2012.08.619